Aside from Spider-Man, characters featured included Beetle, Lizard, Mysterio, Alistair Smythe, Green Goblin, Scorpion, Venom, Doctor Octopus, and Carnage. Between the title screen and the in-game demonstration, a short biography of every character involved in this video game is shown in Japanese along with a suitable background.
Web-swinging abilities are featured on the shoulder buttons; making this feature exclusive to this particular game. Not even other Spider-Man games for the Super NES had the web-swinging abilities as easily accessible as this video game. The right shoulder button swings the forward while the left shoulder button swings the web back. This video game is infamous for timed checkpoints within each level, as players must reach their destination in less than sixty seconds. This prevents players from exploring areas for power-ups and extra lives.
- The games soundtrack is very reminiscent of the Spider-Man animated series of the early 1980s, mainly Spider-Man and His Amazing Friends.
- The option mode is strangely enough in English while the text throughout the entire game is in Japanese except for the quote "He is... Your Friendly Neighborhood Spider-Man."